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Primer sequences for key <t> LncRNA </t> and GAPDH.
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Primer sequences for key  LncRNA  and GAPDH.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Primer sequences for key LncRNA and GAPDH.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Sequencing

Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Construct

The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Expressing, Quantitative RT-PCR, Control

The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Methylation, Control